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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and thus could contribute to tumorigenesis. This protein was located to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital role in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two additional splice variants have been reported but in depth nucleotide sequence information and facts will not be nonetheless offered.
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Blocking peptides are peptides that bind especially to your focus on antibody and block antibody binding. These peptide typically includes the epitope recognized from the antibody.
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This guide outlines more possible optimization of mobile and immunoassay parameters to be certain the absolute best success are...
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This information outlines even more attainable optimization of mobile and immunoassay parameters to make certain the absolute best outcomes are...
Antibodies certain to the blocking peptide no longer bind for the epitope to the focus on protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is specific.
Goal Information Cyclin E belongs on the extremely conserved cyclin household, whose customers are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation patterns which add on the temporal coordination of every mitotic occasion. Cyclin E types a complex with and functions as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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